Tuesday, 9 April 2024

Q. By switching the position of promotor with terminator the definition of coding and template strand could be reversed. How.

 Q. By switching the position of promotor with terminator the definition of coding and template  strand could be reversed. How.

Ans. Switching the position of the promoter and terminator in a DNA sequence would reverse the direction of transcription, which in turn would change which strand serves as the template for mRNA synthesis. Normally, the promoter region is where RNA polymerase binds to initiate transcription, and the terminator signals the end of transcription. 


If you switch their positions, the RNA polymerase would bind at what was previously the terminator and transcribe in the opposite direction, using what was previously the coding (non-template) strand as the template for mRNA synthesis. Consequently, the strand that was previously the coding (non-template) strand would become the template strand, and vice versa, effectively reversing their roles.